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This strain provides an improved Tet-On tool that allows the inducible expression of genes in the developing and adult lung and respiratory epithelium. These transgenic mice express the reverse tetracycline-controlled transactivator rtTA protein under the control of the human SFTPCsurfactant, pulmonary-associated protein C, promoter. In situ hybridization detects rtTA mRNA in lung peripheral epithelial cells from adult mice and 15 postconception day-aged embryos from doxycycline treated dams.
These tetO 27Kip1 mice express a transgene containing the reverse tetracycline-controlled transactivator rtTA protein under the control of the rat nestin promoter that allows the inducible expression of genes in the central nervous system. Mice that are hemizygous for this transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. According to the donating investigator, expression occurs in the neuroepithelium of the developing nervous system embryonic daysin some neuron subsets of the adult mouse e.
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